![]() XLD Agar was developed by Taylor in order to increase the efficiency of the isolation and identification of enteric pathogens, particularly Shigella. Its use is recommended for Salmonella isolation after selenite or tetrathionate enrichment in food analysis both coliforms and Shigella are inhibited. XL Agar Base can be made selective for Salmonella by adding 1.25 mL/L of 1% aqueous brilliant green to the base prior to autoclaving. This medium can be rendered moderately selective for enteric pathogens, particularly Shigella, by the addition of sodium desoxycholate (2.5 g/L) to make XLD Agar. It is particularly recommended for obtaining counts of enteric organisms. XL Agar Base was developed by Taylor1 for the nonselective isolation and differentiation of gram-negative enteric bacilli. Due to the large numbers of different microbial species and strains with varying nutritional requirements and chemical resistance patterns, investigators have developed various formulae to meet general as well as specific needs relative to isolation and identification of the microorganisms. *Adjusted and/or supplemented as required to meet performance criteria.Ī wide variety of media have been developed to aid in the selective isolation and differentiation of enteric pathogens. Reaction of 5.7% (Solution at 25☌): pH 7.4 ± 0.2ĭifco™ XLD Agar - Approximate Formula* Per Liter : Prepared Appearance: Red, slightly opalescent. Solution is red, very slightly to slightly opalescent. Solution: 5.7% solution, soluble in purified water upon boiling. XLD Agar conforms with specifications of The United States Pharmacopeia (USP), is the complete Xylose Lysine Desoxycholate Agar, a moderately selective medium recommended for isolation and differentiation of enteric pathogens, especially Shigella species.ĭehydrated Appearance: Pink, free-flowing, homogeneous. Milieu sélectif pour isolement et la différenciation des espèces pathogènes tel que Shigella et Providencia. ![]()
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